Search for a diagnostic/prognostic biomarker for the brain cancer glioblastoma multiforme by 2D-DIGE-MS technique

The prognosis of patients with glioblastoma multiforme, the most malignant adult glial brain tumor, remains poor in spite of advances in treatment procedures, including surgical resection, irradiation, and chemotherapy. Genetic heterogeneity of glioblastoma warrants extensive studies to gain a thoro...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2012-08, Vol.367 (1-2), p.59-63
Main Authors: Banerjee, Hirendra Nath, Mahaffey, Kelly, Riddick, Eilena, Banerjee, Arnold, Bhowmik, Niladri, Patra, Manomita
Format: Article
Language:English
Subjects:
Astrocytes - metabolism
Biochemistry
Biomarkers
Biomarkers, Tumor - metabolism
Biomedical and Life Sciences
Brain cancer
Brain Neoplasms - diagnosis
Brain Neoplasms - metabolism
Brain tumors
Cancer
Cardiology
Cell Line, Tumor
Chemotherapy
Cholesterol
Criminal investigation
Glioblastoma - diagnosis
Glioblastoma - metabolism
Glioblastoma multiforme
Heterogeneity
Humans
Irradiation
Life Sciences
Mass spectrometry
Medical Biochemistry
Medical diagnosis
Medical prognosis
Methods
Oncology
Peptides
Prognosis
Proteome - metabolism
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Two-Dimensional Difference Gel Electrophoresis
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Summary:The prognosis of patients with glioblastoma multiforme, the most malignant adult glial brain tumor, remains poor in spite of advances in treatment procedures, including surgical resection, irradiation, and chemotherapy. Genetic heterogeneity of glioblastoma warrants extensive studies to gain a thorough understanding of the biology of this tumor. While there have been several studies of global transcript profiling of glioma with the identification of gene signatures for diagnosis and disease management, translation into clinics is yet to happen. In the present study, we report a novel proteomic approach by using two-dimensional difference gel electrophoresis followed by spot picking and analysis of proteins/peptides by Mass spectrometry. We report at least ten different novel proteins/peptides as identified by this technique which are differentially expressed in this cancer and could be of further importance for diagnostic, therapeutic, and prognostic approaches.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-012-1319-6