Induction of chicken cytokine responses in vivo and in vitro by lipooligosaccharide of Campylobacter jejuni HS:10

• Published in: Veterinary microbiology 2013-05, Vol.164 (1-2), p.122-130
• Main Authors: Barjesteh, Neda, Hodgins, Douglas C., St. Paul, Michael, Quinteiro-Filho, Wanderley M., DePass, Christina, Monteiro, Mario A., Sharif, Shayan
• Format: Article
• Language: English
• Subjects: Animals; Campylobacter jejuni; Campylobacter jejuni - immunology; Campylobacter jejuni - physiology; Carbohydrate Sequence; Chicken; Chickens - microbiology; Cytokines; Cytokines - immunology; Female; Humans; Innate immunity; Lipooligosaccharide; Lipopolysaccharides - chemistry; Lipopolysaccharides - immunology; Meat - microbiology; Molecular Sequence Data; Spleen - cytology; Spleen - immunology; Spleen - metabolism
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2013.02.002

Campylobacter jejuni is a pathogen of the gastrointestinal tract of humans, but colonizes chickens for prolonged periods without causing disease. It is unclear what host and bacterial mechanisms maintain a non-inflammatory state in chickens. The present work was undertaken to characterize cytokine responses of chickens to purified lipooligosaccharide (LOS) of C. jejuni HS:10. Chickens were injected with purified LOS, and expression of interleukin (IL)-1β (pro-inflammatory cytokine), IL-8 (pro-inflammatory chemokine), interferon (IFN)γ (Th1-like cytokine), IL-10 (immune regulatory/anti-inflammatory cytokine) and IL-13 (Th2-like cytokine) was evaluated in spleen using quantitative RT-PCR, up to 24h post-injection. In an in vitro study, splenocytes were incubated with LOS, and cytokine expression followed up to 18h. Chickens injected with LOS had increased expression of IL-1β up to 24h later. Expression of IL-8 was significantly increased at 2h but then declined below baseline. Expression of IFNγ and IL-10 was increased significantly at 2h, but declined thereafter. Splenocytes incubated with LOS had increased expression of IL-1β and IL-8 up to 18h of incubation. Expression of IFNγ was increased at 6 and 18h, IL-10 was increased at 2h, but expression of IL-13 did not differ significantly up to 18h. It is concluded that LOS of C. jejuni can induce expression of pro-inflammatory IL-1β and IL-8, as well as IFNγ and IL-10 in chickens. More extensive studies with more prolonged exposure to LOS are needed to further clarify the interaction between C. jejuni and the chicken host.