Acicular, but not globular, titanium dioxide nanoparticles stimulate keratinocytes to produce pro-inflammatory cytokines

Titanium dioxide (TiO2) nanoparticles, widely used for daily products, are believed to be biologically inert, but they may cause adverse effects on cells, presumably depending on the particle size and shape. One of the critical targets of TiO2 particles is epidermal keratinocytes, and their initial...

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Bibliographic Details
Published in:Journal of dermatology 2013-05, Vol.40 (5), p.357-362
Main Authors: Hiroike, Mizuho, Sakabe, Jun-ichi, Kobayashi, Miwa, Shimauchi, Takatoshi, Ito, Taisuke, Hirakawa, Satoshi, Inoh, Akinori, Tokura, Yoshiki
Format: Article
Language:English
Subjects:
Cells, Cultured
cytokine
Cytokines
Humans
Inflammasomes
Interleukin-8 - metabolism
keratinocyte
Keratinocytes - drug effects
Keratinocytes - metabolism
Keratinocytes - ultrastructure
nanoparticle
Nanoparticles
Titanium - pharmacology
titanium dioxide
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Summary:Titanium dioxide (TiO2) nanoparticles, widely used for daily products, are believed to be biologically inert, but they may cause adverse effects on cells, presumably depending on the particle size and shape. One of the critical targets of TiO2 particles is epidermal keratinocytes, and their initial response to TiO2 may be production of pro‐inflammatory cytokines. We therefore investigated the effects of four types of TiO2 particles on cytokine expression/production by real‐time reverse transcription polymerase chain reaction and enzyme‐linked immunoassay. The TiO2 particles included three acicular types, FTL‐100 (length, 1.68 μm; diameter, 130 nm), FTL‐200 (2.86, 210) and FTL‐300 (5.15, 270), and one globular type, PT‐301 (diameter, 270 nm). Normal human epidermal keratinocytes (NHEK) were cultured with each of the TiO2 particles. During cultivation, the acicular forms of TiO2 were seen by scanning electron microscopy to be internalized by NHEK. The three acicular particles increased the mRNA expressions and supernatant productions of interleukin (IL)‐1α, IL‐1β, IL‐6, tumor necrosis factor‐α and IL‐8 in particle number‐dependent manners, whereas globular PT‐301 had very weak activity. Thus, TiO2 particles may induce skin inflammation depending on the size and shape, providing knowledge on their health usage.
ISSN:0385-2407
1346-8138
DOI:10.1111/1346-8138.12132