Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains. e62947

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid...

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Bibliographic Details
Published in:PloS one 2013-05, Vol.8 (5)
Main Authors: Isaksson, Linnea, Mayzel, Maxim, Saline, Maria, Pedersen, Anders, Rosenlow, Joakim, Brutscher, Bernhard, Karlsson, B Goran, Orekhov, Vladislav Y
Format: Article
Language:English
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Summary:We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.
ISSN:1932-6203
DOI:10.1371/journal.pone.0062947