Determination of the genotoxic effects of Convolvulus arvensis extracts on corn (Zea mays L.) seeds

In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75...

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Bibliographic Details
Published in:Toxicology and industrial health 2013-06, Vol.29 (5), p.449-459
Main Authors: Sunar, Serap, Yildirim, Nalan, Aksakal, Ozkan, Agar, Guleray
Format: Article
Language:English
Subjects:
Allelopathy
Biology
Cell division
Convolvulus - chemistry
Convolvulus arvensis
Corn
DNA, Plant - analysis
DNA, Plant - chemistry
DNA, Plant - isolation & purification
Genomic Instability - drug effects
Methanol
Mutagenicity Tests
Mutagens - chemistry
Mutagens - toxicity
Nonnative species
Plant Extracts - chemistry
Plant Extracts - toxicity
Plant Leaves - chemistry
Plant Proteins - analysis
Plant Roots - chemistry
Proteins
Random Amplified Polymorphic DNA Technique
Seeds
Seeds - chemistry
Seeds - drug effects
Toxicology
Variance analysis
Zea mays
Zea mays - chemistry
Zea mays - drug effects
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Summary:In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75 and 100 μl concentrations and applied to corn seed. The total soluble protein and genomic template stability results were compared with the control. The results showed that especially 100 μl extracts of diluted leaf, stem and root had a strong inhibitory activity on the genomic template stability. The changes occurred in random amplification of polymorphic DNA (RAPD) profiles of C. arvensis extract treatment included variation in band intensity, loss of bands and appearance of new bands compared with control. Also, the results obtained from this study revealed that the increase in the concentrations of C. arvensis extract increased the total soluble protein content in maize. The results suggested that RAPD analysis and total protein analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals.
ISSN:0748-2337
1477-0393
DOI:10.1177/0748233712436644