Taxonomic identification of Macrolophus pygmaeus and Macrolophus melanotoma based on morphometry and molecular markers

Two Macrolophus species, M. melanotoma (=M. caliginosus) and M. pygmaeus, have been referred to as efficient predators of several key pests on vegetable crops in Europe. However, due to the great morphological similarity of these species, they have been confused, with important consequences for inoc...

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Bibliographic Details
Published in:Bulletin of entomological research 2013-04, Vol.103 (2), p.204-215
Main Authors: Castañé, C., Agustí, N., Arnó, J., Gabarra, R., Riudavets, J., Comas, J., Alomar, Ó.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Crops
Dittrichia viscosa
Eggs
Female
Flora
Fundamental and applied biological sciences. Psychology
Greenhouses
Hemiptera - anatomy & histology
Hemiptera - classification
Hemiptera - genetics
Host plants
Insecta
Invertebrates
Lycopersicon esculentum
Macrolophus
Male
Males
Mitochondrial DNA
Morphology
Multivariate Analysis
Natural populations
Ovary - cytology
Ovum - cytology
Predators
Principal Component Analysis
Research Paper
Species Specificity
Systematics. Geographical distribution
Taxonomy
Tomatoes
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Summary:Two Macrolophus species, M. melanotoma (=M. caliginosus) and M. pygmaeus, have been referred to as efficient predators of several key pests on vegetable crops in Europe. However, due to the great morphological similarity of these species, they have been confused, with important consequences for inoculative releases of these predators in greenhouses and for the conservation of their natural populations on greenhouse and outdoor crops. In this work, we developed tools to identify these morphologically very similar species. We first confirmed the specific status of two Macrolophus populations collected on their respective host plants (Dittrichia viscosa and tomato) through crossing experiments. Then, using multivariate morphometric analysis, we proposed a linear discriminant function that combines head measurements separating males from the two species without error. Finally, we designed specific primers for a mitochondrial DNA region that were able to distinguish field-collected Macrolophus individuals through conventional PCR. In conclusion, the tools developed in the present study will allow reliable identification of the Macrolophus species present in crops and in the native flora that are the source of populations that colonise them. They will also allow correct identification of mass reared Macrolophus to be introduced in greenhouse crops in inoculative releases.
ISSN:0007-4853
1475-2670
DOI:10.1017/S0007485312000545