Molecular methods for strain typing of Candida albicans: a review

Summary Candida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied microbiology 2013-06, Vol.114 (6), p.1559-1574
Main Authors: Saghrouni, F., Ben Abdeljelil, J., Boukadida, J., Ben Said, M.
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Biological and medical sciences
Candida albicans
Candida albicans - classification
Candida albicans - genetics
Fundamental and applied biological sciences. Psychology
Microbiology
Microsatellite Repeats
Molecular biology
Molecular Typing
Multilocus Sequence Typing
Mycological Typing Techniques
Polymerase Chain Reaction - methods
Polymorphism, Genetic
sequence polymorphisms
strain typing
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Candida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non‐DNA‐based methods (multi‐locus enzyme electrophoresis), conventional DNA‐based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep‐PCR) and DNA‐based methods called exact typing methods because they generate unambiguous and highly reproducible typing data (including microsatellite length polymorphism and multi‐locus sequence typing). In this review, the main molecular methods used for C. albicans strain typing are summarized, and their advantages and limitations are discussed with regard to their discriminatory power, reproducibility, cost and ease of performance.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12132