Bone marrow processing for transplantation using Cobe Spectra cell separator

Abstract Concentration of bone marrow aspirates is an important prerequisite prior to infusion of ABO incompatible allogeneic marrow and prior to cryopreservation and storage of autologous marrow. In this paper we present our experience in processing 15 harvested bone marrow for ABO incompatible all...

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Bibliographic Details
Published in:Transfusion and apheresis science 2013-06, Vol.48 (3), p.359-363
Main Authors: Veljković, Dobrila, Nonković, Olivera Šerbić, Radonjić, Zorica, Kuzmanović, Miloš, Zečević, Željko
Format: Article
Language:English
Subjects:
ABO Blood-Group System
ABO incompatibility
Adolescent
Anemia, Aplastic - therapy
Antigens, CD34 - metabolism
Blood Group Incompatibility
Bone marrow
Bone Marrow - metabolism
Bone Marrow - pathology
Bone Marrow Cells - cytology
Bone Marrow Transplantation - methods
Cell Separation - instrumentation
Cell Separation - methods
Child
Child, Preschool
Cobe Spectra
Erythrocytes - cytology
Female
Health technology assessment
Hematology, Oncology and Palliative Medicine
Humans
Infant
Leukemia, Myeloid, Acute - therapy
Lymphohistiocytosis, Hemophagocytic - therapy
Lymphoma, Non-Hodgkin - therapy
Male
Myelodysplastic Syndromes - therapy
Neuroblastoma - therapy
Precursor Cell Lymphoblastic Leukemia-Lymphoma - therapy
Processing
RBC depletion
Volume reduction
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Summary:Abstract Concentration of bone marrow aspirates is an important prerequisite prior to infusion of ABO incompatible allogeneic marrow and prior to cryopreservation and storage of autologous marrow. In this paper we present our experience in processing 15 harvested bone marrow for ABO incompatible allogeneic and autologous bone marrow (BM) transplantation using Cobe Spectra® cell separator. BM processing resulted in the median recovery of 91.5% CD34+ cells, erythrocyte depletion of 91% and volume reduction of 81%. BM processing using cell separator is safe and effective technique providing high rate of erythrocyte depletion and volume reduction, and acceptable recovery of the CD34+ cells.
ISSN:1473-0502
1878-1683
DOI:10.1016/j.transci.2013.04.017