Antioxidant activity of sugarcane molasses against 2,2′-azobis(2-amidinopropane) dihydrochloride-induced peroxyl radicals

• Published in: Food chemistry 2013-11, Vol.141 (1), p.466-472
• Main Authors: Asikin, Yonathan, Takahashi, Makoto, Mishima, Takashi, Mizu, Masami, Takara, Kensaku, Wada, Koji
• Format: Article
• Language: English
• Subjects: Amidines - chemistry; Amidines - toxicity; Biological and medical sciences; Cell Line; Chemical and cellular antioxidants; DNA Damage - drug effects; DNA damage protection; Food toxicology; Free Radical Scavengers - chemistry; Free Radical Scavengers - pharmacology; Humans; Medical sciences; Molasses - analysis; Peroxides - chemistry; Peroxides - toxicity; Peroxyl radicals; Phenolic constituents; Plant Extracts - chemistry; Plant Extracts - pharmacology; Saccharum - chemistry; Sugarcane molasses; Toxicology
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2013.03.045

•Sugarcane molasses constituents effectively scavenge AAPH-induced peroxyl radicals.•Antioxidative fractions were evaluated via chemical, cellular, and DNA approaches.•The range of ORAC value is 4399–6266μmol TE/g, and EC50 for CAA is 3.7–5.9μg/ml.•Two fractions protect against oxidative DNA damage as potent as gallic acid.•Phenolic constituents were identified in the sugarcane molasses fractions. Sugarcane molasses is a rich source of antioxidant materials with peroxyl radical scavenging effects. To explore the potent antioxidant activity of sugarcane molasses against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced peroxyl radicals, 7 methanolic fractions of sugarcane molasses (F1–F7) were separated via bioassay-guided fractionation and evaluated by oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and oxidative DNA damage protective activity assays. The ORAC values of sugarcane molasses fractions ranged from 4399 to 6266μmol TE/g, whilst the EC50 values for CAA ranged from 3.7 to 5.9μg/ml. Moreover, it was found that sugarcane molasses fractions, particularly F6 and F7, could protect against oxidative DNA damage caused by peroxyl radicals at an effective concentration of 100μg/ml. Ten phenolic constituents were identified in the fractions, including known antioxidative compounds, viz., schaftoside, isoschaftoside, ferulic acid, p-coumaric acid, and p-hydroxybenzaldehyde.