Speciation of selenium in human urine by HPLC-ICP-MS with a collision and reaction cell

A detailed evaluation of the performance of a hexapole collision and reaction cell in inductively coupled plasma mass spectrometry (ICP-MS) for the speciation of selenium in human urine has been carried out. The relative limits of detection, monitoring isotope super(80)Se, were in the range 30-80 pp...

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Bibliographic Details
Published in:Journal of analytical atomic spectrometry 2001-01, Vol.16 (5), p.457-463
Main Authors: Manuel Marchante-Gayón, Juan, Feldmann, Ingo, Thomas, Christoph, Jakubowski, Norbert
Format: Article
Language:English
Subjects:
Body fluids
High performance liquid chromatography
High pressure effects
Inductively coupled plasma
Isotopes
Mass spectrometry
Plasma collision processes
Sulfur compounds
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Summary:A detailed evaluation of the performance of a hexapole collision and reaction cell in inductively coupled plasma mass spectrometry (ICP-MS) for the speciation of selenium in human urine has been carried out. The relative limits of detection, monitoring isotope super(80)Se, were in the range 30-80 ppt (using a hydraulic high pressure nebulizer) and 100-200 ppt (using a Meinhard nebulizer) for the seleno-compounds not interfered by the urinary matrix. Selenium species separation has been carried out by reversed-phase and ion-pair high-performance liquid chromatography (HPLC). Also, the chromatographic behaviour of some sulfur compounds was studied. Unfortunately, the retention times of the selenoaminoacids and the analogue sulfur compounds were not the same, and some chemical interactions between selenite and cysteine or reduced glutathion were observed. The urinary selenium speciation was carried out in 8 different subjects before and after 10 days of supplementation with different commercial nutritional tablets. Trimethylselenonium was found in 3 subjects as one of the main selenium species in urine before and after supplementation. However, a compound, not identified yet, was observed to be the main urinary selenium species after supplementation in all cases.
ISSN:0267-9477
1364-5544
DOI:10.1039/B008649P