Comparison of passive hemagglutination with turkey erythrocyte assay, enzyme-linked immunosorbent assay, and counter-immunoelectrophoresis assay for serological evaluation of tetanus immunity

Antibody titers to tetanus toxin in human sera were assayed by passive hemagglutination with turkey erythrocytes, enzyme-linked immunosorbent assay, and counter-immunoelectrophoresis. The first two of these tests were shown to be the most sensitive for antibody detection, having the same range of se...

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Bibliographic Details
Published in:Journal of clinical microbiology 1983-01, Vol.17 (3), p.432-435
Main Authors: Pitzurra, L, Bistoni, F, Pitzurra, M, Bastianini, L, Perito, S, Vecchiarelli, A, Marconi, P
Format: Article
Language:English
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Summary:Antibody titers to tetanus toxin in human sera were assayed by passive hemagglutination with turkey erythrocytes, enzyme-linked immunosorbent assay, and counter-immunoelectrophoresis. The first two of these tests were shown to be the most sensitive for antibody detection, having the same range of sensitivity and reproducibility. The antibody levels determined by these assays were up to 400-fold higher than those determined by counter-immunoelectrophoresis. The turkey erythrocyte hemagglutination assay requires only 40 min, whereas the immunosorbent assay method requires 24 h. These results suggest that the hemagglutination assay is the more appropriate methods for rapid and sensitive determination of tetanus antibody levels.
ISSN:0095-1137