An HPLC postcolumn reaction system for phosphorus-specific detection in the complete separation of inositol phosphate congeners in aqueous samples

A method for the separation of all the inositol phosphate congeners has been developed and demonstrated. Phosophorus-specific detection is achieved by postcolumn illumination by high-intensity ultraviolet radiation with resultant formation of reduced phosphomolybdate by flow injection of an ascorbic...

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Bibliographic Details
Published in:Environmental science & technology 1992-01, Vol.26 (1), p.199-204
Main Authors: Clarkin, Catherine M, Minear, Roger A, Kim, Seungdo, Elwood, Jerry W
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Ecosystems
Exact sciences and technology
Other chromatographic methods
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Summary:A method for the separation of all the inositol phosphate congeners has been developed and demonstrated. Phosophorus-specific detection is achieved by postcolumn illumination by high-intensity ultraviolet radiation with resultant formation of reduced phosphomolybdate by flow injection of an ascorbic acid/molybdate reagent and spectrophotometric detection.
ISSN:0013-936X
1520-5851
DOI:10.1021/es00025a025