Inhibition of HIV and HSV infection by vaginal lactobacilli in vitro and in vivo

The in vitro anti-viral effect of vaginal and non-vaginal lactobacillus was evaluated using single cycle HIV-1 replication and HSV-2 plaque reduction assays. The XTT proliferation assay was used to monitor the cellular toxicity. The in vivo anti-HSV-1 activity was evaluated in BALB/c mouse model by...

Full description

Saved in:
Bibliographic Details
Published in:Daru 2012-10, Vol.20 (10), p.1-1
Main Authors: Zabihollahi, Rezvan, Motevaseli, Elahe, Sadat, Seyed Mehdi, Azizi-Saraji, Ali Reza, Asaadi-Dalaie, Sogol, Modarressi, Mohammad Hossein
Format: Article
Language:English
Subjects:
HIV
Human immunodeficiency virus
Human immunodeficiency virus 1
Infections
Lactobacillus
Microbiology
Sexually transmitted diseases
STD
Viral infections
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The in vitro anti-viral effect of vaginal and non-vaginal lactobacillus was evaluated using single cycle HIV-1 replication and HSV-2 plaque reduction assays. The XTT proliferation assay was used to monitor the cellular toxicity. The in vivo anti-HSV-1 activity was evaluated in BALB/c mouse model by monitoring skin lesion and immune response development. DMEM culture supernatant of L. Gasseri and L. fermentum (PH 7.3) did not show toxic effects but inhibited 50% of HIV replication at 12% and 31% concentrations, respectively. Co-culture of L. gasseri (1000 CFU/ target cell) showed mild cytotoxicity but inhibited 68% of HIV replication. The supernatant of L. crispatus inhibited 50% of HSV replication at 4% and also co-culture of L. gasseri, L. rhamnosus and L. crispatus revokes almost all of the HSV multiplication. Culture supernatants of L. gasseri and L. crispatus had significant virucidal effect against the HIV and HSV and inhibited HSV infection in a stage before viral entry to the target cells.
ISSN:1560-8115
2008-2231