ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

•Spinal cord injury (SCI) induced ADAMTS expression and proteoglycan fragmentation.•ADAMTS1, 4, 5 and 9, not -8, -15 were expressed in spinal cord tissues in mouse.•Astrocytes are a potential source of ADAMTS in SCI.•This is the first demonstration to target ADAMTS and proteoglycans in mouse SCI. AD...

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Bibliographic Details
Published in:Neuroscience letters 2013-06, Vol.544, p.25-30
Main Authors: Demircan, Kadir, Yonezawa, Tomoko, Takigawa, Tomoyuki, Topcu, Vehap, Erdogan, Serpil, Ucar, Fatma, Armutcu, Ferah, Yigitoglu, M. Ramazan, Ninomiya, Yoshifumi, Hirohata, Satoshi
Format: Article
Language:English
Subjects:
ADAM protein
ADAM Proteins - metabolism
ADAMTS
ADAMTS1 Protein
ADAMTS5 Protein
ADAMTS9 Protein
Aggrecanase
Animals
Astrocytes
Endopeptidases - metabolism
Mice
Mice, Inbred C57BL
Proteoglycans - metabolism
Spinal Cord - metabolism
Spinal Cord Injuries - metabolism
Spinal cord injury
Thoracic Vertebrae - injuries
Thoracic Vertebrae - metabolism
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Summary:•Spinal cord injury (SCI) induced ADAMTS expression and proteoglycan fragmentation.•ADAMTS1, 4, 5 and 9, not -8, -15 were expressed in spinal cord tissues in mouse.•Astrocytes are a potential source of ADAMTS in SCI.•This is the first demonstration to target ADAMTS and proteoglycans in mouse SCI. ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2013.02.064