Stripe rust resistance genes in the UK winter wheat cultivar Claire

• Published in: Theoretical and applied genetics 2013-06, Vol.126 (6), p.1599-1612
• Main Authors: Powell, N. M., Lewis, C. M., Berry, S. T., MacCormack, R., Boyd, L. A.
• Format: Article
• Language: English
• Subjects: Agricultural production; Agriculture; Agriculture - methods; Basidiomycota; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Cultivars; Disease; Disease Resistance - genetics; Diseases and pests; Fungi, Pathogenic; Genes; Genes, Plant - genetics; Genetic aspects; Genetic Markers - genetics; Health aspects; Infections; Life Sciences; Original Paper; Physiological aspects; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plant resistance; Quantitative Trait Loci - genetics; Research parks; Triticum - genetics; Triticum aestivum; United Kingdom; Wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-013-2077-x

Stripe rust resistance in the winter wheat cultivar Claire had remained effective in the UK and Europe since its release in 1999 and consequently has been used extensively in wheat breeding programs. However, in 2012, reports indicated that this valuable resistance may now have been compromised. To characterise stripe rust resistance in Claire and determine which genes may still confer effective resistance a cross was made between Claire and the stripe rust susceptible cultivar Lemhi. A genetic linkage map, constructed using SSR, AFLP, DArT and NBS-AFLP markers had a total map length of 1,730 cM. To improve the definition of two quantitative trait loci (QTL) identified on the long arm of chromosome 2D further markers were developed from wheat EST. Stripe rust resistance was evaluated on adult plants under field and glasshouse conditions by measuring the extent of fungal growth and sporulation, percentage infection (Pi) and the necrotic/chlorotic responses of the plant to infection, infection type (IT). Four QTL contributing to stripe rust adult plant resistance (APR) were identified in Claire, QYr.niab - 2D.1 , QYr.niab - 2D.2, QYr.niab - 2B and QYr.niab - 7B. For Pi QYr.niab - 2D.1 explained up to 25.4 % of the phenotypic variation, QYr.niab - 2D.2 up to 28.7 %, QYr.niab - 2B up to 21.7 % and QYr.niab - 7B up to 13.0 %. For IT the percentages of phenotypic variation explained were 23.4, 31.8, 17.2 and 12.6 %, respectively. In addition to the four QTL conferring APR in Claire, a race-specific, seedling expressed resistance gene was identified on chromosome 3B.