Nanodiamond-Polypyrrole Conductive Composite as Working Electrode for Cholesterol Electrochemical Detection

Enhanced properties of nanocomposites material make them a promising candidate for highly sensitive chemical and biological sensors. Under this study, we have electrochemically synthetized nanodiamond (ND)-polypyrrole (PPy) composite as active substrate for cholesterol oxidase (Chx) enzyme functiona...

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Bibliographic Details
Published in:AIP conference proceedings 2012-03
Main Authors: Villalba, Pedro, Basnayaka, Punya, Ram, Manoj, Kumar, Ashok
Format: Article
Language:English
Subjects:
Cholesterol
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Summary:Enhanced properties of nanocomposites material make them a promising candidate for highly sensitive chemical and biological sensors. Under this study, we have electrochemically synthetized nanodiamond (ND)-polypyrrole (PPy) composite as active substrate for cholesterol oxidase (Chx) enzyme functionalization. ND-PPy/Chx structure was used as a working electrode in three electrode for electrochemical cholesterol detection. The ND-PPy films, before and after enzyme immobilization, were thoroughly characterized by using different techniques. The detection mechanism is based on the production of H2O2 after the reaction of cholesterol molecule in presence of the Chx attached to the sensor surface. During reaction cholesterol is reduced to 4-Cholesten-3-One. Later, the H2O2 molecule is separated into water molecules and free electron that can be amperometrically measured. The calibration curve of cholesterol concentration has been studied using the novel electrode NDPPy films for different known concentration of the analyte under steady state and dynamic conditions.
ISSN:0094-243X