Calibration of a digital in-line holographic microscopy system: depth of focus and bioprocess analysis

Digital in-line holographic microscopy (DIHM) allows access to both intensity and phase information with conventional microscopic lateral resolutions. Such imaging techniques can, however, be used to increase the depth of focus compared to conventional compound microscopes. We present a simple DIHM...

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Bibliographic Details
Published in:Applied optics (2004) 2013-03, Vol.52 (7), p.C78-C87
Main Authors: Ryle, James P, McDonnell, Susan, Glennon, Brian, Sheridan, John T
Format: Article
Language:English
Subjects:
Algorithms
Biopharmaceutics - instrumentation
Biopharmaceutics - methods
Calibration
Cell Line, Tumor
Drug Industry - methods
Equipment Design
Holography - instrumentation
Holography - methods
Humans
Image Processing, Computer-Assisted - methods
Microscopy - instrumentation
Microscopy - methods
Optics and Photonics
Scattering, Radiation
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Summary:Digital in-line holographic microscopy (DIHM) allows access to both intensity and phase information with conventional microscopic lateral resolutions. Such imaging techniques can, however, be used to increase the depth of focus compared to conventional compound microscopes. We present a simple DIHM capable of imaging weakly scattering 10 μm diameter microspheres as well as Hs578T cells over a depth of 1 mm; i.e., we demonstrate an increase by a factor of 100 over the depth of focus of a conventional microscope.
ISSN:1559-128X
2155-3165
DOI:10.1364/AO.52.000C78