Molecularly imprinted polymers on the surface of silica microspheres via sol-gel method for the selective extraction of streptomycin in aqueous samples

• Published in: Journal of separation science 2013-03, Vol.36 (6), p.1142-1148
• Main Authors: Junjie, Li, Mei, Yang, Danqun, Huo, Changjun, Hou, Xianliang, Li, Guomin, Wang, Dan, Feng
• Format: Article
• Language: English
• Subjects: Absorption ability; Adsorption; Analytical chemistry; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Aqueous chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Ethyl alcohol; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Imprinted polymers; Methods of analysis, processing and quality control, regulation, standards; Microspheres; Molecular chemistry; Molecular Imprinting; Molecularly imprinting polymers; Polymers; Polymers - chemical synthesis; Polymers - chemistry; Scanning electron microscopy; Selectivity; Silica; Silicon Dioxide - chemistry; Sol gel process; Sol-gel method; Streptomycin; Streptomycin - chemistry; Streptomycin - isolation & purification; Surface chemistry; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - isolation & purification
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201200869

Streptomycin‐imprinted silica microspheres were prepared by combining a surface molecular‐imprinting technique with the sol‐gel method. A mixture of tetrahydrofuran, ethanol, and water (6:1:1, v/v/v) was selected as dispersing solvent while 3‐aminopropyltriethoxysilane and triethoxyphenylsilane acted as functional monomers, and tetraethyl orthosilicate as a cross‐linker. Characterization of the molecularly imprinted polymers was conducted using scanning electron microscope and dynamic binding experiments. As compared to the nonimprinted polymers, the imprinted polymers exhibited a higher degree of saturated adsorption volume up to 26.3 mg/g, and better selectivity even in an aqueous solution with interfering compounds, including dihydrostreptomycin, neomycin, and tetracycline. The adsorption ability and selectivity were observed to be influenced by the mole ratio of 3‐aminopropyltriethoxysilane and triethoxyphenylsilane. Feasibility of the polymers to be used for actual application was also evaluated with spiked samples, indicating great potential for large‐scale applications. Moreover, the streptomycin‐imprinted polymers can be repeatedly used for 12 cycles without losing original performance, which is beneficial for commercial use.