Treatment with cholesterol-loaded methyl-β-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes

•Treatment with cholesterol-loaded cyclodextrin increased oocyte cholesterol content.•Cyclodextrin acts as a vehicle diffusing cholesterol into the cytoplasm.•Part of this cytoplasmic cholesterol was lost after cryopreservation.•Cholesterol-loaded cyclodextrin did not improve competence of cryoprese...

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Bibliographic Details
Published in:Cryobiology 2013-08, Vol.67 (1), p.106-108
Main Authors: Jiménez-Trigos, E., Vicente, J.S., Mocé, E., Naturil-Alfonso, C., Fernandez-Gonzalez, R., Gutierrez-Adan, A., Marco-Jiménez, F.
Format: Article
Language:English
Subjects:
Animals
Azoles - pharmacology
beta-Cyclodextrins - chemistry
beta-Cyclodextrins - pharmacology
Cholesterol
Cholesterol - chemistry
Cholesterol - pharmacology
Cryopreservation
Cryotop
Cyclodextrin
Female
Nitrobenzenes - pharmacology
Oocytes
Rabbits
Slow-freezing
Sperm Injections, Intracytoplasmic
Vitrification
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Summary:•Treatment with cholesterol-loaded cyclodextrin increased oocyte cholesterol content.•Cyclodextrin acts as a vehicle diffusing cholesterol into the cytoplasm.•Part of this cytoplasmic cholesterol was lost after cryopreservation.•Cholesterol-loaded cyclodextrin did not improve competence of cryopreserved oocytes. Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-β-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2013.05.006