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Malaria vaccine antigen(s): Detergent solubilization, partial isolation, and recovery of immunoprotective activity

Plasmodium berghei-infected erythrocytes were solubilized with nonionic detergent n-octyl glucoside and membrane-containing P. berghei fragments with either n-octyl glucoside or ionic detergent sodium deoxycholate, unsolubilized material was separated by centrifugation and detergent was removed from...

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Published in:Infection and Immunity 1984, Vol.43 (1), p.442-444
Main Authors: D'Antonio, L.E, Keshavarz-Valian, H, Alger, N.E
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Language:English
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container_title Infection and Immunity
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creator D'Antonio, L.E
Keshavarz-Valian, H
Alger, N.E
description Plasmodium berghei-infected erythrocytes were solubilized with nonionic detergent n-octyl glucoside and membrane-containing P. berghei fragments with either n-octyl glucoside or ionic detergent sodium deoxycholate, unsolubilized material was separated by centrifugation and detergent was removed from respective supernatants by gel filtration, reaggregated components in respective void volume eluates acted as specific vaccine in recipient mice
doi_str_mv 10.1128/iai.43.1.442-444.1984
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subjects Animals
antigens
Antigens, Surface - immunology
Antigens, Surface - isolation & purification
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Detergents
Erythrocytes - physiology
Female
Fundamental and applied biological sciences. Psychology
Human protozoal diseases
immunity
immunization
Infectious diseases
Malaria
Malaria - immunology
Malaria - prevention & control
Medical sciences
Mice
Mice, Inbred A
Parasitic diseases
Plasmodium berghei
Plasmodium berghei - pathogenicity
Protozoa
Protozoal diseases
Solubility
Tropical medicine
Vaccines
title Malaria vaccine antigen(s): Detergent solubilization, partial isolation, and recovery of immunoprotective activity
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