Association of Cytokeratin p39 with DNA in Intact Novikoff Hepatoma Cells

The relationship between the rat tumor-associated cytokeratin p39 (Mr39,000) and cellular DNA has been studied in intact cells. Using a DNA-protein crosslinking technique, incubation with cis-dichlorodiammineplatinum, we present evidence for the association of p39 with DNA in intact Novikoff ascites...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (2), p.419-423
Main Authors: Ward, W. S., Schmidt, W. N., Schmidt, C. A., Hnilica, L. S.
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens, Neoplasm - metabolism
Cell Nucleus - ultrastructure
Cellular immunity
Centrifugation
Chromatin
Cisplatin
Crosslinking
DNA
DNA - metabolism
Histones
Histones - metabolism
Intermediate Filament Proteins - metabolism
Irradiation
Keratins
Keratins - metabolism
Liver Neoplasms, Experimental - metabolism
Molecular Weight
Protein Binding
Rats
Spleen cells
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Summary:The relationship between the rat tumor-associated cytokeratin p39 (Mr39,000) and cellular DNA has been studied in intact cells. Using a DNA-protein crosslinking technique, incubation with cis-dichlorodiammineplatinum, we present evidence for the association of p39 with DNA in intact Novikoff ascites hepatoma cells. The cells were treated with cis-dichlorodiammineplatinum and solubilized in NaDodSO4-containing buffer, and the DNA was pelleted by high-speed centrifugation. By immunotransfer analysis, the cytokeratin was found in the DNA pellet of the crosslinked samples while absent from the controls. This result was further substantiated by CsCl density-gradient centrifugation. Collectively, these results suggest a cytokeratin-DNA association at the filament binding sites on or near the nuclear lamina.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.2.419