Further evidence for dolichyl phosphate‐mediated glycosyl translocation through membranes

Mannosyl residues from GDP‐[14C]Man are transferred into liposomes and onto internal GDP if the liposomal membranes contain dolichyl phosphate and the enzyme GDP‐Man: Dol‐P mannosyl transferase. Experiments using a mixture of GDP‐[14C]Man and [3H]GDP‐Man excluded the possibility that the transmembra...

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Bibliographic Details
Published in:FEMS microbiology letters 1984-01, Vol.21 (3), p.305-308
Main Authors: Haselbeck, Anton, Tanner, Widmar
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell physiology
dolichol
Fundamental and applied biological sciences. Psychology
Glycoprotein
liposomes
mannose
Membrane and intracellular transports
membranes
Molecular and cellular biology
Saccharomyces cerevisiae
sugar
sugar transport
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Summary:Mannosyl residues from GDP‐[14C]Man are transferred into liposomes and onto internal GDP if the liposomal membranes contain dolichyl phosphate and the enzyme GDP‐Man: Dol‐P mannosyl transferase. Experiments using a mixture of GDP‐[14C]Man and [3H]GDP‐Man excluded the possibility that the transmembrane translocation of [14C]mannose is due to a GDP‐[14C]Manoutside ag GDPinside exchange reaction.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1984.tb00327.x