Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

• Published in: Journal of general virology 1984-03, Vol.65 (3), p.539-547
• Main Authors: Garcia-Luque, I, Kaper, J. M, Diaz-Ruiz, J. R, Rubio-Huertos, M
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cucumber mosaic virus; Fundamental and applied biological sciences. Psychology; Microbiology; Morphology, structure, chemical composition, physicochemical properties; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Systematics. Structure, properties and multiplication. Genetics; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-65-3-539

1 Plant Virology Laboratory, Plant Protection Institute, A.R.S., U.S.D.A., Beltsville Agricultural Research Center, Beltsville, Maryland 20705, U.S.A. and 2 U.E.I. Virología Vegetal, Instituto ‘Jaime Ferrán’ de Microbiología, C.I.B., C.S.I.C., Joaquín Costa 32, Madrid-6, Spain Satellite RNAs of CARNAs 5s (cucumber mosaic virus-associated RNA 5s) emerged in serial passage lines of Spanish cucumber mosaic virus isolates established in Chenopodium , squash and two tobacco varieties. No satellite was detected in virus obtained from tomato serial passage lines. The satellites were identified by their biological effect on tomato, by their gel electrophoretic mobility and by nucleic acid hybridization, as being very similar in sequence to known CARNA 5s. Interactions among host, virus and CARNA 5 affect the selection of a particular CARNA 5 variant. Keywords: satellite RNAs, cucumoviruses, cDNA-RNA hybridization Received 27 June 1983; accepted 9 November 1983.