Na super(+), Li super(+), and Cl super(-) transport by brush border membranes from rabbit jejunum

Na super(+), Li super(+), K super(+), Rb super(+), Br super(-), Cl super(-) and SO sub(4)u2 super(-) transport were studied in brush border membrane vesicles isolated from rabbit jejunum. Li super(+) uptakes were measured by flameless atomic absorption spectroscopy, and all others were measured usin...

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Bibliographic Details
Published in:The Journal of membrane biology 1983-01, Vol.74 (2), p.85-94
Main Authors: Gunther, R D, Wright, E M
Format: Article
Language:English
Subjects:
brush border membranes
jejunum
rabbits
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Summary:Na super(+), Li super(+), K super(+), Rb super(+), Br super(-), Cl super(-) and SO sub(4)u2 super(-) transport were studied in brush border membrane vesicles isolated from rabbit jejunum. Li super(+) uptakes were measured by flameless atomic absorption spectroscopy, and all others were measured using isotopic flux and liquid scintillation counting. All uptakes were performed with a rapid filtration procedure. A method is presented for separating various compounts of ion uptake: passive diffusion, mediated transport and binding. It was concluded that a Na super(+)/H super(+) exchange mechanism exists in the jejunal brush border.
ISSN:0022-2631