Relative stability of guanosine-cytidine diribonucleotide cores: A super(1)H NMR assessment

Proton NMR was used to study the secondary structure and melting behavior of six self-complementary oligoribonucleotide tetramers each containing two guanosine and two cytidine residues (GGCC, CCGG, GCCG, CGGC, GCGC, and CGCG). GGCC and CCGG formed perfect duplexes containing four G multiplied by C...

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Bibliographic Details
Published in:Biochemistry (Easton) 1984-01, Vol.23 (12), p.2656-2662
Main Authors: Sinclair, A, Alkema, D, Bell, R A, Coddington, J M, Hughes, D W, Neilson, T, Romaniuk, P J
Format: Article
Language:English
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Summary:Proton NMR was used to study the secondary structure and melting behavior of six self-complementary oligoribonucleotide tetramers each containing two guanosine and two cytidine residues (GGCC, CCGG, GCCG, CGGC, GCGC, and CGCG). GGCC and CCGG formed perfect duplexes containing four G multiplied by C base pairs with T sub(m)s of 54 and 47.8 degree C respectively; GCCG and CGGC formed staggered duplexes with two G multiplied by C base pairs and four 3' double-dangling bases, with Ts) of 35.5 and 29.2 degree C, respectively; GCGC formed a perfect duplex with a T sub(m) of 49.9 degree C, while CGCG formed a staggered duplex with a T sub(m) of 36.9 degree C. From these results, an order of stability of the cores containing two G multiplied by C base pairs was proposed: GC:GC is more stable than GG:CC which is more stable than CG:CG.
ISSN:0006-2960