Analytical peptide mapping by ion-exchange high-performance liquid chromatography: application to haemoglobin variants

High-performance liquid chromatography (HPLC) has become increasingly important in the study of amino acid sequences in proteins. A number of advantages of HPLC include convenience and efficiency, high sensitivity, and ready application to both analytical and preparative purposes. Although peptide s...

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Bibliographic Details
Published in:Journal of Chromatography A 1984, Vol.305 (2), p.456-460
Main Authors: Imamura, Takashi, Sugihara, Jun, Yokota, Eisuke, Kagimoto, Masaaki, Naito, Yasushi, Yanase, Toshiyuki
Format: Article
Language:English
Subjects:
amino acid sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
hemoglobin
Hemoproteins
ion exchange
man
Metalloproteins
Proteins
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Summary:High-performance liquid chromatography (HPLC) has become increasingly important in the study of amino acid sequences in proteins. A number of advantages of HPLC include convenience and efficiency, high sensitivity, and ready application to both analytical and preparative purposes. Although peptide separations have been commonly made by HPLX with a reversed-phase column, the use of an ion-exchange column provides a new dimension. This report describes the authors' experiences with separation of tryptic peptides of haemoglobins (Hb) by the use of an ion-exchange column and phosphate-acetonitrile gradient elution. The results indicate that this procedure is a useful technique for routine peptide mapping.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)83361-7