Structure and thermotropic properties of mixed-chain phosphatidylcholine bilayer membranes

The structure and thermotropic properties of hydrated 1-myristoyl-2-palmotoyl-L-phosphatidylcholine (MPPC) and 1-palmitoyl-2-myristoyl-L-phosphatidylcholine (PMPC) have been studied by X-ray diffraction and differential scanning calorimetry (DSC). After prolonged storage at -3 degree C, hydrated mul...

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Bibliographic Details
Published in:Biochemistry (Easton) 1984-01, Vol.23 (4), p.713-720
Main Authors: Serrallach, E. N, De Haas, G. H, Shipley, G. G
Format: Article
Language:English
Subjects:
Artificial membranes and reconstituted systems
Biological and medical sciences
differential scanning calorimetry
Fundamental and applied biological sciences. Psychology
Membrane physicochemistry
Molecular biophysics
phosphatidylcholine
X-ray diffraction
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Summary:The structure and thermotropic properties of hydrated 1-myristoyl-2-palmotoyl-L-phosphatidylcholine (MPPC) and 1-palmitoyl-2-myristoyl-L-phosphatidylcholine (PMPC) have been studied by X-ray diffraction and differential scanning calorimetry (DSC). After prolonged storage at -3 degree C, hydrated multilamellar dispersions of both MPPC and PMPC show two endothermic transitions of comparable enthalpies on initial heating. For both MPPC and PMPC, only the higher temperature transition is readily reversible; the enthalpy associated with the lower temperature transition is progressively recovered with increasing incubation time at low temperatures, suggesting conversions, albeit kinetically complex, between metastable and stable phases. X-ray diffraction data show the presence of bilayer structures and a similar pattern of structural changes on heating for hydrated (50 wt %) MPPC and PMPC.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00299a020