Reconstitution of 50S ribosomal subunits from Bacillus stearothermophilus with 5S RNA from spinach chloroplasts and low-M sub(r) RNA from mitochondria of Locusta migratoria) and bovine liver

Reconstitution experiments with 50S ribosomal subunits from Bacillus stearothermophilus demonstrate that spinach chloroplast 5 S rRNA can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5S rRNA. In contra...

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Bibliographic Details
Published in:FEBS letters 1984-01, Vol.169 (1), p.67-72
Main Authors: Vogel, D W, Hartmann, R K, Bartsch, M, Subramanian, A R, Kleinow, W, O'Brien, T W, Pieler, T, Erdmann, V A
Format: Article
Language:English
Subjects:
Bacillus stearothermophilus
Locusta migratoria
Spinacea oleracea
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Summary:Reconstitution experiments with 50S ribosomal subunits from Bacillus stearothermophilus demonstrate that spinach chloroplast 5 S rRNA can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5S rRNA. In contrast, mitochondria from Locusta migratoria or bovine liver do not appear to contain discrete, low-M sub(r) RNAs, which can replace 5S rRNA in the functional reconstitution of B. stearothermophilus) ribosomes.
ISSN:0014-5793