Lack of beta sub(2)-microglobulin on the surface of canine transmissible venereal tumor cells

beta -Microglobulin ( beta sub(2)m) expression on the cell surface of the naturally occurring, allotransplantable canine transmissible venereal tumor (TVT) was investigated by use of indirect membrane immunofluorescence and radioimmunoassay. Two cell populations were identified in animal-derived, co...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1984-01, Vol.72 (2), p.395-401
Main Authors: Cohen, D, Shalev, A, Krup, M
Format: Article
Language:English
Subjects:
beta 2-microglobulin
canine transmissible venereal tumor
cell surface
dogs
viruses
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Summary:beta -Microglobulin ( beta sub(2)m) expression on the cell surface of the naturally occurring, allotransplantable canine transmissible venereal tumor (TVT) was investigated by use of indirect membrane immunofluorescence and radioimmunoassay. Two cell populations were identified in animal-derived, collagenase disaggregated TVT cell suspensions. About 80% of the cells lacked surface beta sub(2)m expression, whereas about 20% of the cells strongly reacted with anti-dog beta sub(2)m serum. With the use of a cell separation technique, beta sub(2)m-negative cells were demonstrated to carry TVT markers on their surface, whereas the beta sub(2)m-positive cells did not express the tumor markers. The beta sub(2)m-positive cells seemed, therefore, to be tumor-infiltrating host cells. The findings were supported by fluorescence staining studies of frozen sections of the TVT. The lack of beta sub(2)m expression on the surface of TVT cells might explain the allotransplantability of this neoplasm, since beta sub(2) expression on the cell surface appeared to be obligatory for the expression of class 1 major histocompatability complex antigens.
ISSN:0027-8874