Antigenic relatedness of immunoglobulins to Legionella pneumophila serogroups 1-6 from humans and the nonhuman primate Macaca fascicularis

The purpose of this study was to determine whether cynomolgus monkey antisera to L. pneumophila serogroups 1-6 antigens could be used as positive controls in the indirect immunofluorescence assay (IFA) for legionellosis. Immunoelectrophoretic mobilities and Ouchterlony analyses with heavy chain-spec...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 1984-01, Vol.10 (5), p.261-264
Main Authors: BRAKE, B. J, WILKINSON, H. W, GELOK, R, MARKOVITS, A
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Legionella pneumophila
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The purpose of this study was to determine whether cynomolgus monkey antisera to L. pneumophila serogroups 1-6 antigens could be used as positive controls in the indirect immunofluorescence assay (IFA) for legionellosis. Immunoelectrophoretic mobilities and Ouchterlony analyses with heavy chain-specific antisera and IFA titers with immunoglobulin class-specific conjugates were used to show antigenic relatedness of immunized monkey immunoglobulins to those produced as a result of infection in humans. Identical immunoelectrophoretic precipitation patterns were obtained for human and monkey sera with antihuman gamma, mu, and alpha heavy-chain-specific antisera. These data suggest that hyperimmune cynomolgus monkey antisera are suitable alternatives to human sera for IFA-positive controls.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01577138