Effect of storage time on haematological parameters in mullet, Mugil cephalus

The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin co...

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Bibliographic Details
Published in:Cell biochemistry and function 2013-07, Vol.31 (5), p.412-416
Main Authors: Faggio, C., Casella, S., Arfuso, F., Marafioti, S., Piccione, G., Fazio, F.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Automation, Laboratory
Blood Platelets - chemistry
Blood Platelets - cytology
Erythrocyte Count
Erythrocyte Indices
Erythrocytes - chemistry
Erythrocytes - cytology
haematological automatic analysis
haematological parameters
Hematocrit
Leukocyte Count
Leukocytes - chemistry
Leukocytes - cytology
Male
Mugil cephalus
Platelet Count
Refrigeration - standards
Reproducibility of Results
Smegmamorpha - blood
Specimen Handling - standards
storage time
teleost
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Summary:The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P 
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.2915