Effect of temperature and pH on the hopanoid content of Bacillus acidocaldarius

A gas‐liquid chromatographic method for the quantitative determination of hopanoids with an elongated side chain was developed. After extraction of lipids from Bacillus acidocaldarius, periodate oxidation, reduction and acetylation the 1‐hydroxyethane‐29‐hopane acetate was quantitated by comparison...

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Bibliographic Details
Published in:FEMS microbiology letters 1984-01, Vol.23 (2‐3), p.253-256
Main Authors: Poralla, Karl, Härtner, Thomas, Kannenberg, Elmar
Format: Article
Language:English
Subjects:
Bacillus acidocaldarius
Bacteriology
Biological and medical sciences
cytoplasmic membranes
extreme environments
Fundamental and applied biological sciences. Psychology
Hopanoids
Microbiology
Morphology, structure, chemical composition
temperature
temperature adaptation
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Summary:A gas‐liquid chromatographic method for the quantitative determination of hopanoids with an elongated side chain was developed. After extraction of lipids from Bacillus acidocaldarius, periodate oxidation, reduction and acetylation the 1‐hydroxyethane‐29‐hopane acetate was quantitated by comparison with 1‐octadecanyl‐glycerol ether. The contents of extended hopanoids increased strongly with increasing temperature and moderately with decreasing pH. Hopene showed no significant alteration with temperature and pH.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1984.tb01073.x