Cloning and identification of the H-2D super(p) gene

The authors have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2D super(p)-specific monoclonal antibodies. The cell line L12a did not react with K super(p)-specific monoclonal antibod...

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Bibliographic Details
Published in:Immunogenetics (New York) 1984-01, Vol.19 (3), p.194-204
Main Authors: Macchi, MJ, Woodward, J G, McLaughlin-Taylor, E, Griffin, J, Hood, L, Frelinger, JA
Format: Article
Language:English
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Summary:The authors have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2D super(p)-specific monoclonal antibodies. The cell line L12a did not react with K super(p)-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2 super(p). Only a single 8.8 kb Bam H1 fragment can be assigned to K super(p) by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.
ISSN:0093-7711