A semi-continuous process for the production of human interferon-αc from E. coli using tangential-flow microfiltration and immuno-affinity chromatography

A semi-continuous process for the production of human interferon- alpha c (IFN) from E. coli is described. Harvesting of bacteria, removal of cell debris and concentration were performed by tangetial-flow microfiltration (cross-flow microfiltration). Purification was done in a single step by a monoc...

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Bibliographic Details
Published in:Biotechnology letters 1984-01, Vol.6 (10), p.621-626
Main Authors: VAKS, B, MORY, Y, PEDERSON, J. U, HOROVITZ, O
Format: Article
Language:English
Subjects:
affinity chromatography
alpha -interferon
Antiviral drugs
Applied microbiology
Biological and medical sciences
Biotechnology
Escherichia coli
filtration
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
man
Microbiology
Miscellaneous
Production of active biomolecules
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Summary:A semi-continuous process for the production of human interferon- alpha c (IFN) from E. coli is described. Harvesting of bacteria, removal of cell debris and concentration were performed by tangetial-flow microfiltration (cross-flow microfiltration). Purification was done in a single step by a monoclonal-Ab column. In the process, purified material, 3-9 x 10 super(8) units/mg protein was obtained with a 65% overall yield.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00133826