A rapid method for separating phosphorus compounds by molecular weight using exclusion gels and centrifugation

The separation of the various phosphorus compounds present in lakewaters is needed to assess their availability and role in algal assemblages. Separating these compounds by gel chromatography can take several hours, and denaturation can occur during that time. It is possible to accelerate the proced...

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Bibliographic Details
Published in:Water research (Oxford) 1984, Vol.18 (9), p.1181-1183
Main Authors: Brassard, P., Auclair, J.C.
Format: Article
Language:English
Subjects:
Analytical chemistry
batch
centrifuge
Chemistry
Chromatographic methods and physical methods associated with chromatography
chromatography
Exact sciences and technology
extraction
gel
method
phosphorus
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Description
Summary:The separation of the various phosphorus compounds present in lakewaters is needed to assess their availability and role in algal assemblages. Separating these compounds by gel chromatography can take several hours, and denaturation can occur during that time. It is possible to accelerate the procedure by using several batch extractions in sequence. The method described allows the simultaneous analysis of 4 samples in about 20 min. This gain in time is obtained at the expense of resolution since the whole elution profile is spread over 10 samples. This method was found simple and efficient in applications where the speed of separation is more important than resolution.
ISSN:0043-1354
1879-2448
DOI:10.1016/0043-1354(84)90236-7