Somatic hybridisation using a double mutant of Nicotiana tabacum

Summary Leaf mesophyll protoplasts of a nitrate reductase deficient, streptomycin resistant double mutant of Nicotiana tabacum were fused with cell suspension protoplasts of wild type N. rustica . Hybrid colonies were selected for nitrate reductase proficiency and streptomycin resistance. Green, act...

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Bibliographic Details
Published in:Heredity 1984-01, Vol.53 (1), p.79-83
Main Authors: Pental, D, Hamill, J.D, Cocking, E.C
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cell cultures. Hybridization. Fusion
Cytogenetics
Ecology
Economic plant physiology
Evolutionary Biology
Fundamental and applied biological sciences. Psychology
Human Genetics
In vitro culture
Molecular and cellular biology
Nicotiana rustica
Nicotiana tabacum
nitrate reductase
original-article
plant breeding
Plant cells and fungal cells
plant genetics
Plant Genetics and Genomics
plantlets
protoplast fusion
streptomycin
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Summary:Summary Leaf mesophyll protoplasts of a nitrate reductase deficient, streptomycin resistant double mutant of Nicotiana tabacum were fused with cell suspension protoplasts of wild type N. rustica . Hybrid colonies were selected for nitrate reductase proficiency and streptomycin resistance. Green, actively proliferating colonies were recovered in the selection medium. Eleven of twenty green colonies, transferred to regeneration medium, produced plants. Regenerated plants were analysed for their vegetative and floral characteristics, Fraction 1 protein polypeptide composition and leaf esterases. All the eleven regenerants were nuclear somatic hybrids possessing the chloroplast of the N. tabacum parent. By developing a double mutant with a negative (auxotrophic) and a positive (resistance) selection marker in a species of interest, this species can be hybridised by somatic cell fusion with wild type species lacking selectable markers.
ISSN:0018-067X
1365-2540
DOI:10.1038/hdy.1984.64