Some mitogens cause rapid increases in free calcium in fibroblasts

• Published in: FEBS letters 1984-04, Vol.169 (2), p.189-193
• Main Authors: Morris, Jonathan D.H., Metcalfe, James C., Smith, Gerry A., Hesketh, T.Robin, Taylor, Michael V.
• Format: Article
• Language: English
• Subjects: 3T3 fibroblast; Aminoquinolines - metabolism; Animals; Biological and medical sciences; Calcium - metabolism; Cell cycle, cell proliferation; Cell Line; Cell physiology; Dinoprost; Epidermal Growth Factor - pharmacology; Fibroblasts - drug effects; Fibroblasts - metabolism; Free Ca 2; Free Ca2; Fundamental and applied biological sciences. Psychology; Growth factor; Insulin - pharmacology; Mice; Mitogenic stimulation; Mitogens - pharmacology; Molecular and cellular biology; Prostaglandins F - pharmacology; Quin2; Tetradecanoylphorbol Acetate - pharmacology; Vasopressins - pharmacology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(84)80316-6

Quiescent 3T3 fibroblasts grown on microcarrier beads and loaded with the [Ca 2+] indicator quin2 had a cytosolic free Ca 2+ concentration ([Ca 2+] i) of 154 ± 11 nM (SE; n = 32). Stimulation with the mitogens vasopressin, epidermal growth factor (EGF) or prostaglandin F 2α (PGF 2α) caused a very rapid increase in [Ca 2+] i to a maximum of 200–500 nM after 60–90 s. [Ca 2+] i declined thereafter to a level above that in quiescent cells which was maintained for at least 15 min. In contrast no immediate effects on [Ca 2+] i were detected after the addition of the mitogens insulin or 12- O-tetradecanoylphorbol 13-acetate (TPA). These studies indicate that early changes in [Ca 2+] i may be involved in the action on fibroblasts of some, but not all, mitogens.