The inhibitor ribonucleoprotein of poly(A)-containing non-polysomal messenger ribonucleoprotein of A. salina cryptobiotic embryos

The inhibitor present in poly(A)-containing non-polysomal mRNP preparations of A. salina cryptobiotic embryos has been purified by sucrose density gradient centrifugation, gel filtration on Sephacryl S200 and ion-exchange chromatography on DEAE-cellulose. The inhibitor is a ribonucleoprotein sedimen...

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Bibliographic Details
Published in:FEBS letters 1984-09, Vol.175 (1), p.16-20
Main Authors: Piot, Etienne, Backhovens, Hubert, Slegers, Herman
Format: Article
Language:English
Subjects:
Artemia salina
Biological and medical sciences
DTT, dithiothreitol
embryos
Fundamental and applied biological sciences. Psychology
Hepes, 4-(2-hydroxyethyl)-l-piperazine-ethane sulfonic acid
iRNA, inhibitor ribonucleic acid
iRNP
iRNP, inhibitor ribonucleoprotein
Molecular and cellular biology
Molecular genetics
mRNP
mRNP, messenger ribonucleoprotein
PMSF, phenylmethylsulfonyl fluoride
Protein synthesis
ribonucleoproteins
Translation. Translation factors. Protein processing
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Summary:The inhibitor present in poly(A)-containing non-polysomal mRNP preparations of A. salina cryptobiotic embryos has been purified by sucrose density gradient centrifugation, gel filtration on Sephacryl S200 and ion-exchange chromatography on DEAE-cellulose. The inhibitor is a ribonucleoprotein sedimenting at 5–6 S with a M r, of ~ 84 000 ± 6000. The particle is composed of a protein with M r of 64 000 and an RNA with a length of 85 ± 2 nucleotides and is a very potent inhibitor of the translation of mRNA. The inhibition is a property of the RNA but the inhibitor ribonucleoprotein is more efficient in the inhibition of poly(A) containing mRNA than its extracted RNA.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80560-8