Modulated anisotropy fluorescence for quantitative determination of carbaryl and benomyl

Two individual components in mixtures have been resolved by frequency domain fluorescence technique by measuring the observable quantities which characterize the anisotropy decay; differential anisotropy phase and modulated anisotropy ratio (MAR), which in turn are related to the rotational correlat...

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Bibliographic Details
Published in:Talanta (Oxford) 2003-06, Vol.60 (2), p.629-634
Main Authors: NAVAS DIAZ, A, GARCIA SANCHEZ, F, LOPEZ GUERRERO, M. M
Format: Article
Language:English
Subjects:
Analytical chemistry
Benomyl
Carbaryl
Chemistry
Dynamic anisotropy
Exact sciences and technology
Phase-modulation fluorescence
Small molecules
Spectrometric and optical methods
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Description
Summary:Two individual components in mixtures have been resolved by frequency domain fluorescence technique by measuring the observable quantities which characterize the anisotropy decay; differential anisotropy phase and modulated anisotropy ratio (MAR), which in turn are related to the rotational correlation time. The method presented here is capable of directly resolving binaries mixtures of fluorophores on the basis of differences in their rotational diffusion rates. Our results demonstrate that modulation anisotropy ratio measurements can be used for quantitative determination of small analytes, carbaryl and benomyl, having identical or nearly identical fluorescence spectra. This methodology can be applied with good results when the fluorophores have a suitable MAR difference.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(03)00101-2