Culturing Primary Human Osteoblasts on Electrospun Poly(lactic-co-glycolic acid) and Poly(lactic-co-glycolic acid)/Nanohydroxyapatite Scaffolds for Bone Tissue Engineering

• Published in: ACS applied materials & interfaces 2013-07, Vol.5 (13), p.5921-5926
• Main Authors: Li, Mengmeng, Liu, Wenwen, Sun, Jiashu, Xianyu, Yunlei, Wang, Jidong, Zhang, Wei, Zheng, Wenfu, Huang, Deyong, Di, Shiyu, Long, Yun-Ze, Jiang, Xingyu
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - chemistry; Bone Development; Cell Proliferation; Cell Survival; Durapatite - chemistry; Humans; Lactic Acid - chemistry; Mice; Osteoblasts - cytology; Osteoblasts - metabolism; Osteocalcin - metabolism; Polyglycolic Acid - chemistry; Primary Cell Culture; Tissue Engineering - instrumentation; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am401937m

In this work, we fabricated polymeric fibrous scaffolds for bone tissue engineering using primary human osteoblasts (HOB) as the model cell. By employing one simple approach, electrospinning, we produced poly(lactic-co-glycolic acid) (PLGA) scaffolds with different topographies including microspheres, beaded fibers, and uniform fibers, as well as the PLGA/nanohydroxyapatite (nano-HA) composite scaffold. The bone-bonding ability of electrospun scaffolds was investigated by using simulated body fluid (SBF) solution, and the nano-HA in PLGA/nano-HA composite scaffold can significantly enhance the formation of the bonelike apatites. Furthermore, we carried out in vitro experiments to test the performance of electrospun scaffolds by utilizing both mouse preosteoblast cell line (MC 3T3 E1) and HOB. Results including cell viability, alkaline phosphatase (ALP) activity, and osteocalcin concentration demonstrated that the PLGA/nano-HA fibers can promote the proliferation of HOB efficiently, indicating that it is a promising scaffold for human bone repair.