In vitro antiproliferative effect of a water-soluble Laminaria japonica polysaccharide on human melanoma cell line A375

• Published in: Food and chemical toxicology 2013-08, Vol.58, p.56-60
• Main Authors: Peng, Zhenfei, Liu, Min, Fang, Zhexiang, Chen, Li, Wu, Jiulin, Zhang, Qiqing
• Format: Article
• Language: English
• Subjects: A375 cells; anticarcinogenic activity; Antitumor activity; Apoptosis; Bcl-2 family proteins; bcl-2-Associated X Protein - metabolism; Biological and medical sciences; Cell Line, Tumor; Cell Proliferation - drug effects; Humans; Laminaria - chemistry; Laminaria japonica polysaccharide; Medical sciences; melanoma; Melanoma - metabolism; Melanoma - pathology; Membrane Potential, Mitochondrial - drug effects; mitochondrial membrane; Polysaccharides - pharmacology; proteins; Saccharina japonica; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2013.04.026

•A polysaccharide WPS-2-1 was purified from Laminaria japonica.•WPS-2-1 exhibited high inhibitory effect on the A375 cells proliferation.•WPS-2-1 induced apoptosis in A375 cells with the increase in Bax/Bcl-2 ratio.•WPS-2-1 induced apoptosis via mitochondrial apoptotic pathway. A water-soluble polysaccharide WPS-2-1, purified from Laminaria japonica, has been found to have antitumor activity. In this study, WPS-2-1 exhibited high anti-proliferative activity on A375 cells in a dosedependent manner. Further investigation indicated that WPS-2-1 induced A375 cells apoptosis. Moreover, WPS-2-1-induced apoptosis was associated with the alteration in expressions of Bcl-2 family proteins. Mitochonadrial apoptotic pathway was involved in WPS-2-1-induced apoptosis, which included the loss of mitochondrial membrane and activation of caspase-3/9. The results in this study suggested that WPS-2-1 could effectively inhibit proliferation of A375 cells in vitro and induce apoptosis via mitochondrial apoptotic pathway. It might serve as a potential antitumor agent.