ApoE3 mediated polymeric nanoparticles containing curcumin: Apoptosis induced in vitro anticancer activity against neuroblastoma cells

• Published in: International journal of pharmaceutics 2012-11, Vol.437 (1-2), p.29-41
• Main Authors: Mulik, Rohit S., Mönkkönen, Jukka, Juvonen, Risto O., Mahadik, Kakasaheb. R., Paradkar, Anant R.
• Format: Article
• Language: English
• Subjects: anticarcinogenic activity; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Apolipoprotein E3; Apolipoprotein E3 - administration & dosage; Apolipoprotein E3 - chemistry; apoptosis; Apoptosis - drug effects; Caspase 3 - metabolism; Cell Cycle - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Curcumin; Curcumin - administration & dosage; Curcumin - chemistry; Drug Carriers - administration & dosage; Drug Carriers - chemistry; Enbucrilate - administration & dosage; Enbucrilate - chemistry; Flow cytometry; Humans; in vitro studies; Ligands; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; neoplasms; nervous system diseases; Neuroblastoma; neurosecretory system; photostability; Reactive Oxygen Species - metabolism; zeta potential
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2012.07.062

Curcumin, a natural phytoconstituent, is known to be therapeutically effective in the treatment of various cancers such as, breast cancer, lung cancer, pancreatic cancer, brain cancer, etc. However, low bioavailability and photodegradation of curcumin hampers its overall therapeutic efficacy. Anionic polymerization method was employed for the preparation of apolipoprotein-E3 mediated curcumin loaded poly(butyl)cyanoacrylate nanoparticles (ApoE3-C-PBCA) and characterized for size, zeta potential, entrapment efficiency, photostability, morphology, and in vitro release study. ApoE3-C-PBCA were found to be effective against SH-SY5Y neuroblastoma cells compared to curcumin solution (CSSS) and curcumin loaded PBCA nanoparticles (C-PBCA) from in vitro cell culture investigations. Flow cytometry techniques employed for the detection of anticancer activity revealed enhanced activity of curcumin against SH-SY5Y neuroblastoma cells with ApoE3-C-PBCA compared to CSSS and C-PBCA, and apoptosis being the underlying mechanism. Present study revealed that ApoE3-C-PBCA has tremendous potential to develop into an effective therapeutic treatment modality against brain cancer.