The mechanism of ORFH79 suppression with the artificial restorer fertility gene Mt-GRP162

The restoration fertility complex (RFC) was previously identified in Honglian (HL)-cytoplasmic male sterility (CMS) rice (Oryza sativa), and glycine-rich protein 162 (GRP162) is responsible for binding to the CMS-associated transcript atp6-orfH79. Here, we engineered a recombinant GRP162 containing...

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Bibliographic Details
Published in:The New phytologist 2013-07, Vol.199 (1), p.52-58
Main Authors: Hu, Jun, Huang, Wenchao, Huang, Qi, Qin, Xiaojian, Dan, Zhiwu, Yao, Guoxin, Zhu, Renshan, Zhu, Yingguo
Format: Article
Language:English
Subjects:
Cytoplasmic male sterility
cytoplasmic male sterility (CMS)
Cytotoxicity
Fertility
Gene Expression Regulation, Plant
Genes
Glycine
Glycine (amino acid)
glycine‐rich protein (GRP)
Honglian (HL)
Male fertility
Male sterility
Mitochondria
Mitochondria - genetics
Mitochondria - metabolism
mitochondrion
Open Reading Frames
Oryza - genetics
Oryza - physiology
Oryza sativa
Plant fertility
Plant Infertility - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified - genetics
Pollen
Pollen - genetics
Proteins
Rapid reports
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombinants
Restoration
restoration of fertility complex (RFC)
Rice
RNA
RNA, Plant - genetics
RNA, Plant - metabolism
Sterility
Transcription
Transgenic plants
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Summary:The restoration fertility complex (RFC) was previously identified in Honglian (HL)-cytoplasmic male sterility (CMS) rice (Oryza sativa), and glycine-rich protein 162 (GRP162) is responsible for binding to the CMS-associated transcript atp6-orfH79. Here, we engineered a recombinant GRP162 containing the mitochondrial transit peptide, termed Mt-GRP162, as an artificial restorer of fertility (Rf) gene. Mt-GRP162 was confirmed to bind to CMS-associated RNA and to localize to the mitochondria. The transgenic plants showed restored fertility with partially functional pollen. Wefound that the expression of ORFH79 decreased in transgenic plants, while the expression of atp6-orfH79 was not changed. These findings indicate that Mt-GRP162 restores fertility by suppressing the expression of the cytotoxic protein ORFH79 at the post-transcriptional level rather than via the cleavage of atp6-orfH79 in the presence of RFC. These findings contribute to our understanding of the mechanisms of restoration through diverse pathways.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.12310