Biological Pretreatment of Rice Straw by Phenarocheate chrysosporium for the Production of Cellulases and Xylanases using Asperigillus niger Isolate

• Published in: Research journal of microbiology 2012-01, Vol.7 (1), p.1-1
• Main Authors: Mohan, P R, Ramesh, B, Reddy, O V S
• Format: Article
• Language: English
• Subjects: Oryza sativa
• ISSN: 1816-4935; 2151-8211

Rice straw was pretreated biologically with Phenarocheate chrysosporium and subsequently used in the production of cellulases and xylanases employing Asperigillus niger isolate. The FTIR spectrum of pretreated rice straw was compared with standard cellulose library. The spectra of 1048 cm-1 for CoO stretch in cellulose and hemicellulose were observed in rice straw. The enzymatic activity produced by A. niger on biologically Pretreated Rice Straw (PRS) was higher than that of the control treatments, which were carried out with Untreated Rice Straw (URS) and Cellulose (CEL). For medium supplemented with PRS, the peak activities obtained were 1.03 plus or minus 0.06, 1.54 plus or minus 0.08, 0.82 plus or minus 0.14 and 1.02 plus or minus 0.08 U mL... for FPase, endoglucanase, p-glucosidases and xylanases, respectively. For the cellulose control, values of 0.68 plus or minus 0.04, 1.24 plus or minus 0.11, 0.54 plus or minus 0.16 and 0.90 plus or minus 0.06 U mL... and URS values of 0.48 plus or minus 0.08, 0.94 plus or minus 0.1O, 0.46 plus or minus 0.12 and 0.64 plus or minus 0.14 U mL... were obtained, respectively. This was substantiated by higher contents of N-acetylglucosamine and fungal protein in PRS samples than the cellulose control and untreated rice straw samples. (ProQuest: ... denotes formulae/symbols omitted.)