Characterization of the transporterB super(0)AT3 (Slc6a17) in the rodent central nervous system

• Published in: BMC neuroscience 2013-01, Vol.14 (1), p.54-54
• Main Authors: Haegglund, Maria GA, Hellsten, Sofie V, Bagchi, Sonchita, Ljungdahl, Anna, Nilsson, Victor CO, Winnergren, Sonja, Stephansson, Olga, Rumaks, Juris, Svirskis, Simons, Klusa, Vija, Schioth, Helgi B, Fredriksson, Robert
• Format: Article
• Language: English
• ISSN: 1471-2202; 1471-2202
• DOI: 10.1186/1471-2202-14-54

Background: The vesicular B super(0)AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B super(0)AT3 in mouse brain using in situ hybridization and immunohistochemistry. Results: We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B super(0)AT3 was highly expressed in both inhibitory and excitatory neurons. The B super(0)AT3 expression was highly overlapping with those of vesicular glutamate transporter 2 (VGLUT2) and vesicular glutamate transporter 1 (VGLUT1). We also show here that Slc6a17mRNA is up-regulated in animals subjected to short term food deprivation as well as animals treated with the serotonin reuptake inhibitor fluoxetine and the dopamine/noradrenaline reuptake inhibitor bupropion. Conclusions: This suggests that the B super(0)AT3 transporter have a role in regulation of monoaminergic as well as glutamatergic synapses.