Halomonas socia sp. nov., isolated from high salt culture of Dunaliella salina

• Published in: Extremophiles : life under extreme conditions 2013-07, Vol.17 (4), p.663-668
• Main Authors: Cao, Jiao, Ma, Huai-Yuan, Li, Hai-Yu, Wang, Kui-Rong, Ruan, Kun, Bai, Lin-Han
• Format: Article
• Language: English
• Subjects: Bacteria; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chlorophyta - microbiology; Deoxyribonucleic acid; DNA; Dunaliella salina; Genes, Bacterial - genetics; Genotype & phenotype; Gram-negative bacteria; Halomonas; Halomonas - genetics; Halomonas - isolation & purification; Halomonas - metabolism; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Phylogeny; RNA, Ribosomal, 16S - genetics; Salinity; Salt; Sodium chloride; Space life sciences; Taxonomy
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s00792-013-0549-1

A moderately halophilic bacteria designed strain NY-011 T was isolated from the high salt culture of Dunaliella salina in Chengdu of Sichuan Province, China. The isolate was Gram-negative, nonmotile, rod-shaped and 12.5–21.6 μm in length. Colonies on solid media are circular, wet, smooth and cream. The strain grew optimally at 37 °C, pH 7.0 and in the presence of 8 % NaCl. Acid was produced from glycerol, d -arabinose, glucose, trehalose, inositol, mannose, mannitol, sucrose, maltose and sorbitol. Catalase is produced but not oxidase. The major fatty acids are C18: 1ω7c (37.59 %), C19: 0 cyclo ω8c (18.29 %), C16: 0 (16.05 %) and C6: 0 (12.43 %). The predominant respiratory lipoquinone found in strain NY-011 T is ubiquinone with nine isoprene units (Q-9). The genomic DNA G + C content of strain NY-011 T was 62.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NY-011 T belonged to the genus Halomona s. The highest levels of 16S rRNA gene sequence similarity were found between the strain NY-011 T and H. pantelleriensis (sequence similarity 98.43 %). However, the levels of DNA–DNA relatedness between them were only 23.1 %. In addition, the strain NY-011 T had a phenotypic profile that readily distinguished it from H. pantelleriensis. The strain NY-011 T therefore represents a new species of the genus Halomonas , for which the name Halomonas socia sp. nov. is proposed, with NY-011 T (=CCTCC AB 2011033 T  = KCTC 23671 T ) as the type strain.