Recurrent Pregnancy Loss, Plasminogen Activator Inhibitor-1 (-675) 4G/5G Polymorphism and Antiphospholipid Antibodies in Czech Women

Problem This study compares the frequencies of plasminogen activator inhibitor‐1 (‐675) 4G/5G polymorphism and its relationship with eight antiphospholipid antibodies (aPLs) in serum of 157 patients with repeated pregnancy loss (RPL). Method of study PAI‐1 (‐675) 4G/5G polymorphism was determined us...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2013-07, Vol.70 (1), p.54-58
Main Authors: Subrt, Ivan, Ulcova-Gallova, Zdenka, Cerna, Monika, Hejnalova, Marketa, Slovanova, Jitka, Bibkova, Katarina, Micanova, Zdenka
Format: Article
Language:English
Subjects:
Abortion, Habitual - blood
Abortion, Habitual - genetics
Abortion, Habitual - immunology
Adult
Antibodies, Antiphospholipid - blood
Antiphospholipid antibodies
Czech Republic
European Continental Ancestry Group - genetics
Female
Gene Frequency
Genotype
Genotype & phenotype
Humans
Miscarriage
PAI-1
Plasminogen Activator Inhibitor 1 - genetics
Polymorphism, Genetic
Pregnancy
pregnancy loss
thrombophilia
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Summary:Problem This study compares the frequencies of plasminogen activator inhibitor‐1 (‐675) 4G/5G polymorphism and its relationship with eight antiphospholipid antibodies (aPLs) in serum of 157 patients with repeated pregnancy loss (RPL). Method of study PAI‐1 (‐675) 4G/5G polymorphism was determined using standard PCR‐RFLP method. Enzyme‐linked immunosorbent assay was used for the detection of aPLs against ph‐serine, ph‐ethanolamine, ph‐inositol, ph‐DL‐glycerol, phosphatidic acid, annexin V, cardiolipin, and beta2‐GPI. Allelic frequency and distribution of genotypes were calculated. The prevalence of the risk conferring 4G allele and 4G/4G homozygous genotype in patients and controls was compared, and the correlation between aPLs positivity and PAI‐1 4G/4G genotype was tested by chi‐square test. Results Statistically highly significant correlation between RPL and PAI‐1 (‐675) 4G/4G genotype was found. No correlation between PAI‐1 (‐675) 4G/5G polymorphism and the presence of antiphospholipid antibodies in RPL patients was observed. Conclusions PAI‐1 (‐675) 4G/4G homozygous genotype increases the risk of RPL independently from the aPLs positivity.
ISSN:1046-7408
1600-0897
DOI:10.1111/aji.12099