Sensitive profiling of biogenic amines in human urine by capillary electrophoresis with field amplified sample injection

• Published in: Biomedical chromatography 2013-08, Vol.27 (8), p.987-993
• Main Authors: Bacaloni, A., Insogna, S., Sancini, A., Ciarrocca, M., Sinibaldi, F.
• Format: Article
• Language: English
• Subjects: Acetonitriles; biogenic amines; Biogenic Amines - isolation & purification; Biogenic Amines - urine; capillary electrophoresis; Electrophoresis, Capillary - methods; field-amplified sample injection; Humans; Hydrogen-Ion Concentration; Limit of Detection; Linear Models; Reproducibility of Results; urine; work-related stress biomarkers
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.2891

ABSTRACT In order to monitor biogenic amines in human urine, a method based on field‐amplified sample injection combined with capillary electrophoresis and direct UV absorption detection was developed. Dopamine, tyramine, tryptamine, serotonin and epinephrine were effectively separated and identified in human urine samples, and detection limits were 0.072, 0.010, 0.027, 0.010 and 0.120 µmol/L, respectively. Detection limits comparable to laser‐induced fluorescence detection or solid phase extraction combined with capillary electrophoresis were achieved. Parameters affecting electrophoretic system detection sensitivity were investigated. Optimal separation conditions were obtained using as background electrolyte a pH 6.5 mixture of 2‐(morpholino)ethanesulfonic acid 20 mmol/L and 30 mmol/L phosphate buffer, containing 0.05% hydroxypropylcellulose and 10% v/v methanol. Injections of the sample solution were performed by applying a voltage of 12 kV for 50 s. Recovery and accuracy ranged between 89.4 and 94.9%, and 89 and 112%, respectively. The method was successfully applied on actual urine samples (from a healthy volunteer): target bioamine content was consistent with endogenous levels reported in the literature. The proposed method is simple, fast and inexpensive and can be conveniently employed in work‐related stress studies. The affordability and noninvasive sampling of the method allow epidemiological studies on large number of exposed persons to be performed. Copyright © 2013 John Wiley & Sons, Ltd.