Collagen-hydroxyapatite/Cisplatin Drug Delivery Systems for Locoregional Treatment of Bone Cancer

• Published in: Technology in cancer research & treatment 2013-08, Vol.12 (4), p.275-284
• Main Authors: Andronescu, Ecaterina, Ficai, Anton, Albu, Madalina Georgiana, Mitran, Valentina, Sonmez, Maria, Ficai, Denisa, Ion, Raluca, Cimpean, Anisoara
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents - administration & dosage; Bone Neoplasms - drug therapy; Bone Neoplasms - pathology; Cell Line, Tumor; Cisplatin - administration & dosage; Collagen - administration & dosage; Drug Delivery Systems; Durapatite - administration & dosage; Humans; Porosity; Proliferating Cell Nuclear Antigen - analysis; Spectrophotometry, Infrared; X-Ray Diffraction
• ISSN: 1533-0346; 1533-0338
• DOI: 10.7785/tcrt.2012.500331

In this paper, the synthesis and characterization of novel cisplatin-loaded collagen (COLL)/hydroxyapatite (HA) composite materials are presented. The composite materials were designed to obtain a COLL: HA weight ratio close to the bone composition. The content of embedded cisplatin was chosen to assure a concentration of cisplatin of 6 and 10 μM, respectively, into the culture media used in cell culture experiments. These cisplatin delivery systems were characterized by determining the physico-chemical properties of the composite material, the drug release process as well as their biological activity. Based on the in vitro data that showed the cytotoxic, anti-proliferative and anti-invasive activities of these multifunctional systems on G292 osteosarcoma cells in dependence on the cisplatin concentration released in culture medium, we conclude that the newly developed COLL/HA-cisplatin drug delivery system could be a feasible approach for locoregional chemotherapy of bone cancer.