Structure and gene cluster of the O-antigen of Escherichia coli O76

The O-polysaccharide (O-antigen) of Escherichia coli O76 was studied by sugar analysis along with 1D and 2D 1H,13C NMR spectroscopies. The following structure of the linear tetrasaccharide repeating unit was established: [Display omitted] The degree of O-acetylation of 4-substituted β-GalNAc residue...

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Bibliographic Details
Published in:Carbohydrate research 2013-08, Vol.377, p.14-17
Main Authors: Perepelov, Andrei V., Wang, Quan, Senchenkova, Sof’ya N., Feng, Lu, Shashkov, Alexander S., Wang, Lei, Knirel, Yuriy A.
Format: Article
Language:English
Subjects:
Acetylation
Acetylgalactosamine - chemistry
Bacterial polysaccharide structure
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Base Sequence
Carbohydrate Sequence
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - genetics
Lipopolysaccharide
Magnetic Resonance Spectroscopy
Molecular Sequence Annotation
Molecular Sequence Data
Multigene Family
nuclear magnetic resonance spectroscopy
O Antigens - chemistry
O Antigens - genetics
O-Antigen
O-Antigen gene cluster
Sequence Analysis, DNA
sugars
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Summary:The O-polysaccharide (O-antigen) of Escherichia coli O76 was studied by sugar analysis along with 1D and 2D 1H,13C NMR spectroscopies. The following structure of the linear tetrasaccharide repeating unit was established: [Display omitted] The degree of O-acetylation of 4-substituted β-GalNAc residue is ∼70%. The O-antigen gene cluster of E. coli O76 was sequenced. The functions of genes in the O-antigen gene cluster were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O76 O-antigen structure.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2013.04.027